Composite

Part:BBa_K3802003:Design

Designed by: Heera Bandi, Joelle El Hamouche   Group: iGEM21_Stony_Brook   (2021-09-30)


GSH/T25 Fusion Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1157
    Illegal BamHI site found at 1369
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 209
    Illegal NgoMIV site found at 1508
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PgsA is not orginally from E.coli, but rather from Bacillus subtilis. Therefore, we had to codon optimize the entire part to be compatible with E.coli.


Source

This part is a composite of multiple parts from the registry. It was synthesized by a gene synthesizing compnay (IDT).

References